9th Annual BMRP Investigator Meeting - Abstract
Circulating MicroRNAs in Inflammatory Bowel Disease: Can They Provide Insights into Disease Pathogenesis and Might They Have Diagnostic Utility?
Geoffrey Krissansen1,a, Alan Fraser2,3, Fiona McQueen1,4, Yi Yang1, Megan Williams4, John Egbuji1, Yih-Chih Chan1, Euphemia Leung1, Nicola Dalbeth2 andMark Lane3
Departments of 1Molecular Medicine & Pathology and 2Medicine, Faculty of Medical and Health Sciences, University of Auckland (Auckland, New Zealand); 3Auckland Gastroenterology Associates, Epsom (Auckland, New Zealand); 4District Health Board Rheumatology Services, Greenlane Clinical Centre (Auckland, New Zealand)
Micro-RNAs (miRNAs), once referred to as the biological equivalent of “dark matter”, serve as a second layer of genetic regulation that acts to negatively regulate gene expression, impacting on almost every known biological process. They circulate in the blood, representing a window on our status of health. We sought to determine whether serum miRNAs might serve as diagnostic and prognostic markers of Crohn’s disease (CD), and ulcerative colitis (UC). Peripheral blood was obtained from patients with active CD (confined to the colon), active UC, and from active rheumatoid arthritis patients and normal healthy adults (control groups). MiRNAs in serum samples from 10 subjects in each participant group were assessed and compared by miRNA microarray analysis. Additional serum samples are being collected to enable validation of results. Pooled serum samples are to be analyzed by “deep sequencing” in order to identify potentially novel miRNAs associated with inflammatory bowel disease.
a Principal Investigator