9th Annual BMRP Investigator Meeting - Abstract

Lifestyle-Associated Alterations of Intestinal Microbiota and Their Potential Role in Etiology of Inflammatory Bowel Diseases: A Metagenomic Analysis

Ateequr Rehman1, Limas Kupcinskas2, Aida Zwirbliene2, Deepak Amarapurkar3, Stefan Schreiber1,4 and Stephan J. Ott1,4,a


1
Institute for Clinical Molecular Biology (ICMB), Christian-Albrechts-University (CAU) Kiel (Kiel, Germany); 2Department of Gastroenterology, Kaunas University Hospital (Kaunas, Lithuania); 3Ameya RBI Employees Co-Op Housing Society (Mumbai, India); 4Clinic for Internal Medicine I, University-Hospital Schleswig-Holstein (UK S-H), Campus Kiel (Kiel, Germany)

Bacterial 16S rRNA gene signatures from 90 colonic biopsy specimens of patients with Crohn´s disease (CD), ulcerative colitis (UC) and healthy individuals from three different areas of the world (Germany, Lithuania, India) have been investigated using bar coded second generation high-throughput sequencing with a total of more than 600.000 sequences generated to compare different lifestyles and environments and their potential role for etiology of inflammatory bowel diseases (IBD). The three different regions represent western urban (high incidence and prevalence of IBD) and rural (low incidence and prevalence of IBD) lifestyle as well as a completely different socio-cultural and nutritional (i.e, vegetarian) conditions (very low incidence and prevalence of IBD). In the final analysis the Indian populations showed microbial composition of the intestinal microbiota distinct from the other areas regardless, whether they are healthy or had CD or UC. In detail, we found significant differences in the representation of major phyla and also of bacterial genera, especially Bacteroides and Prevotella, as well as in diversity. From the data, indirect conclusions can be made that specific bacterial populations might be protective towards development of IBD. Others, such as Bacteroides, seem to be harmful for the intestinal homeostasis.

a
Principal Investigator

 

Last updated 05/11/2011