9th Annual BMRP Investigator Meeting - Abstract
Detection of Campylobacter Concisus in Adult Patients with Inflammatory Bowel Disease and Controls
Li Zhang1,a, Stephen Riordan2, Michael Grimm3 and Hazel Mitchell1
1The School of Biotechnology and Biomolecular Sciences, University of New South Wales (Sydney, Australia); 2Gastrointestinal and Liver Unit, The Prince of Wales Hospital and Faculty of Medicine, University of New South Wales (Sydney, Australia); 3St George Clinical School, University of New South Wales (Sydney, Australia)
Previously, we detected a significantly higher prevalence of Campylobacter concisus in intestinal biopsies of children with newly diagnosed Crohn’s disease (CD) as compared with the controls. In the current study, we have examined the presence of C. concisus in adult patients with inflammatory bowel disease (IBD) and controls.
Intestinal biopsies were collected from 15 patients with CD, 6 patients with Ulcerative colitis (UC) and 31 controls. Four biopsies (ileum, caecum, descending colon and rectum) were collected from each individual and used for DNA extraction and detection of C. concisus by PCR. An additional biopsy collected from the caecum was used for cultivation of Campylobacter species.
A significantly higher prevalence of C. concisus was detected in intestinal biopsies from patients with both newly diagnosed CD (33%) and relapsed CD (43%) as compared to controls (16%) (p<0.05). The prevalence of C. concisus in biopsies from patients with UC (30%) was not significantly different from that of the controls (p>0.05).
a Principal Investigator